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KMID : 0357419950250010051
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Korean journal of Virology
1995 Volume.25 No. 1 p.51 ~ p.58
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Propagation of Varicella-Zooster Virus in Serum-Free Medium
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전복환
박호선/최윤/황규계/우규진/정현용/박증석/박송용
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Abstract
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A human embryonic lung fibroblast (LuMA) cell line has been established for production of a live attenuated varicella vaccine. We have investigated the possibility of using serum-free medium for growth of LuMA cells and propagation of both
cell-associated and cell-free varicellazoster virus (VZV). The medium was supplemented with serum-free supplements (HTE:human serum albumin, insulin, transferrin, and monoethanolamine) in a mixture of basal serum-free medium (a mixture of DME and
Ham's
F12 medium, 1:1). We have also investigated the effects of lipid supplementation and low serum addition for promotion of virus propagation in serum-free medium supplemented with HITE. Although the serum-free medium was not adequate for LuMA cell
growth,
the medium supplemented with concentrated lipid emulsion(CLE) was comparable to DME medium supplemented with 2% fetal bovine serum (FBS) in respect of VZV propagation.
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KEYWORD
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